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qPCR is cost-successful and available as the laboratory configurations, machinery and reagents are comparable to Those people wanted for getting ready the NGS libraries.

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It ought to be mentioned that relative and complete abundances based upon 16S rRNA gene copies certainly are a proxy for microbial density as opposed to correct numeration of cells, since 16S rRNA gene duplicate figures vary among microbes. It can be, on the other hand, probable to computationally correct for 16S rRNA gene duplicate figures submit hoc

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The 331F/797R primers were being decided on to the quantification of overall microbes, since the primers goal the V3-V4 hypervariable locations as in Illumina MiSeq. An index of primers and references used in the present review is summarized in S1 Desk.

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